intact e. coli j5 lps  (List Biological Laboratories)

Journal: Journal of the American Society for Mass Spectrometry

Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

doi: 10.1007/s13361-018-1897-y

Figure Lengend Snippet: Agonists were cultured with HEK-Blue hTLR4 cells over a 5-log dose range from 0.1–1000 ng mL−1. W3110 E. coli LPS (red), J5 E. coli LPS (orange), J5 dLPS (pink), J5 dLPS/GBOMP (green), or PHAD (blue) were incubated for 16 hours. Then NF-κB activation was measured by quantification of SEAP in the supernatant. Mean ± SD of duplicate samples and an associated 4-parameter non-linear regression are shown

Article Snippet: Multi-stage MS (MS n ) to confirm structural inferences Intact E. coli J5 LPS from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO) were directly infused in a solution composed of 50% 2-propanol and 50% water at a flow rate of 2 μL min −1 into a home-built nESI source of a linear ion trap (linear trapping quadrupole; LTQ) mass spectrometer (Thermo Finnigan, San Jose, CA) with post-production ion funnel optics added for increased ion transmission efficiency.

Techniques: Cell Culture, Incubation, Activation Assay

Journal: Journal of the American Society for Mass Spectrometry

Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

doi: 10.1007/s13361-018-1897-y

Figure Lengend Snippet: Averaged IMS-CID tandem mass spectra of (a) J5 LPS m/z 1071 and (b) J5 dLPS m/z 789 after collision energy ramping. Insets show deprotonated fatty acid product ions’ presence in (a) and absence in (b)

Article Snippet: Multi-stage MS (MS n ) to confirm structural inferences Intact E. coli J5 LPS from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO) were directly infused in a solution composed of 50% 2-propanol and 50% water at a flow rate of 2 μL min −1 into a home-built nESI source of a linear ion trap (linear trapping quadrupole; LTQ) mass spectrometer (Thermo Finnigan, San Jose, CA) with post-production ion funnel optics added for increased ion transmission efficiency.

Techniques:

Journal: Journal of the American Society for Mass Spectrometry

Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

doi: 10.1007/s13361-018-1897-y

Figure Lengend Snippet: Zoomed negative mode FT-ICR mass spectrum (R ~ 300,000 FWHM, in absorption mode) after direct infusion of J5 LPS. Eight potential isotopic distribution envelopes can be identified in absorption mode in this 4 m/z window; these are denoted, at the m/z of their respective monoisotopic ions, with blue arrows. (inset) Magnified portion of the spectrum showing fine detail (including the magnitude mode and the proposed overlap between isotopologues from envelopes 2 and 5)

Article Snippet: Multi-stage MS (MS n ) to confirm structural inferences Intact E. coli J5 LPS from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO) were directly infused in a solution composed of 50% 2-propanol and 50% water at a flow rate of 2 μL min −1 into a home-built nESI source of a linear ion trap (linear trapping quadrupole; LTQ) mass spectrometer (Thermo Finnigan, San Jose, CA) with post-production ion funnel optics added for increased ion transmission efficiency.

Techniques:

Journal: Journal of the American Society for Mass Spectrometry

Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

doi: 10.1007/s13361-018-1897-y

Figure Lengend Snippet: MS3 CID mass spectra from MS2 product ions representing J5 E. coli lipid A at m/z 1796 (top) and core OS at m/z 1418 (bottom). Similar dissociation phenomena were observed as in MS2 experiments for chemically isolated lipid A and oligosaccharides, indicating feasibility of LPS top down sequencing in this manner

Article Snippet: Multi-stage MS (MS n ) to confirm structural inferences Intact E. coli J5 LPS from both List Biological Laboratories, Inc. (Campbell, CA) and Sigma Aldrich (St. Louis, MO) were directly infused in a solution composed of 50% 2-propanol and 50% water at a flow rate of 2 μL min −1 into a home-built nESI source of a linear ion trap (linear trapping quadrupole; LTQ) mass spectrometer (Thermo Finnigan, San Jose, CA) with post-production ion funnel optics added for increased ion transmission efficiency.

Techniques: Isolation, Sequencing

Journal: Journal of the American Society for Mass Spectrometry

Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

doi: 10.1007/s13361-018-1897-y

Figure Lengend Snippet: Agonists were cultured with HEK-Blue hTLR4 cells over a 5-log dose range from 0.1–1000 ng mL−1. W3110 E. coli LPS (red), J5 E. coli LPS (orange), J5 dLPS (pink), J5 dLPS/GBOMP (green), or PHAD (blue) were incubated for 16 hours. Then NF-κB activation was measured by quantification of SEAP in the supernatant. Mean ± SD of duplicate samples and an associated 4-parameter non-linear regression are shown

Article Snippet: Intact E. coli J5 LPS from Sigma Aldrich (St. Louis, MO), dissolved in a solution of 50% 2-propanol and 50% water at an estimated total LPS concentration of 50 μg mL −1 , was directly infused through a home-built nano-electrospray ionization (nESI) source at a flow rate of 1 μL min −1 into a hybrid linear ion trap – 21 Tesla Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometer, described in detail in a previous publication [ 35 ].

Techniques: Cell Culture, Incubation, Activation Assay

Journal: Journal of the American Society for Mass Spectrometry

Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

doi: 10.1007/s13361-018-1897-y

Figure Lengend Snippet: Averaged IMS-CID tandem mass spectra of (a) J5 LPS m/z 1071 and (b) J5 dLPS m/z 789 after collision energy ramping. Insets show deprotonated fatty acid product ions’ presence in (a) and absence in (b)

Article Snippet: Intact E. coli J5 LPS from Sigma Aldrich (St. Louis, MO), dissolved in a solution of 50% 2-propanol and 50% water at an estimated total LPS concentration of 50 μg mL −1 , was directly infused through a home-built nano-electrospray ionization (nESI) source at a flow rate of 1 μL min −1 into a hybrid linear ion trap – 21 Tesla Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometer, described in detail in a previous publication [ 35 ].

Techniques:

Journal: Journal of the American Society for Mass Spectrometry

Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

doi: 10.1007/s13361-018-1897-y

Figure Lengend Snippet: Zoomed negative mode FT-ICR mass spectrum (R ~ 300,000 FWHM, in absorption mode) after direct infusion of J5 LPS. Eight potential isotopic distribution envelopes can be identified in absorption mode in this 4 m/z window; these are denoted, at the m/z of their respective monoisotopic ions, with blue arrows. (inset) Magnified portion of the spectrum showing fine detail (including the magnitude mode and the proposed overlap between isotopologues from envelopes 2 and 5)

Article Snippet: Intact E. coli J5 LPS from Sigma Aldrich (St. Louis, MO), dissolved in a solution of 50% 2-propanol and 50% water at an estimated total LPS concentration of 50 μg mL −1 , was directly infused through a home-built nano-electrospray ionization (nESI) source at a flow rate of 1 μL min −1 into a hybrid linear ion trap – 21 Tesla Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometer, described in detail in a previous publication [ 35 ].

Techniques:

Journal: Journal of the American Society for Mass Spectrometry

Article Title: Top down tandem mass spectrometric analysis of a chemically modified rough-type lipopolysaccharide vaccine candidate

doi: 10.1007/s13361-018-1897-y

Figure Lengend Snippet: MS3 CID mass spectra from MS2 product ions representing J5 E. coli lipid A at m/z 1796 (top) and core OS at m/z 1418 (bottom). Similar dissociation phenomena were observed as in MS2 experiments for chemically isolated lipid A and oligosaccharides, indicating feasibility of LPS top down sequencing in this manner

Article Snippet: Intact E. coli J5 LPS from Sigma Aldrich (St. Louis, MO), dissolved in a solution of 50% 2-propanol and 50% water at an estimated total LPS concentration of 50 μg mL −1 , was directly infused through a home-built nano-electrospray ionization (nESI) source at a flow rate of 1 μL min −1 into a hybrid linear ion trap – 21 Tesla Fourier transform-ion cyclotron resonance (FT-ICR) mass spectrometer, described in detail in a previous publication [ 35 ].

Techniques: Isolation, Sequencing